Design, optimization, fabrication and analysis of Cu microheater for loop-mediated isothermal amplification (LAMP) applications

Narayanamurthy, Vigneswaran and Samsuri, Fahmi and Jeroish, Z. E. and Shankar, Abhishek Futane and Shanmugavel, Bhuvaneshwari Kumaravel (2023) Design, optimization, fabrication and analysis of Cu microheater for loop-mediated isothermal amplification (LAMP) applications. Materials Today Communications, 36. pp. 1-13. ISSN 2352-4928

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Abstract

A paper presents the design, fabrication, and thermal evaluation of a printed circuit board (PCB) based copper (Cu) microheater that can be integrated with microfluidic chips to initiate the loop-mediated isothermal amplification (LAMP). A series of 3D finite element electro-thermal simulations were carried out to analyze the thermal uniformity and power consumption of the micro heater. The optimal design was fabricated using the etching technique and analyzed with a heat spreader to enhance thermal uniformity. The simulation results of the microheater reveal that the meander configuration outperforms other designs. In addition, the microheater with a heat spreader has a thermal difference of only < 5 ℃ when compared with ~10 ℃ in a microheater without a heat spreader. The developed microheater has a long shelf life and can be used to handle wet biological samples when encapsulated with polyethylene terephthalate (PET). The paper microfluidic chip on the glass substrate has a temperature difference of only 0.5 ℃. The low-cost microheater integrated microfluidic chips has the great potential to develop inexpensive home-based diagnostic kits and trigger the access of diagnostic kits in underdeveloped countries to reduce the spread of infection and initiate treatment plans.

Item Type:	Article
Uncontrolled Keywords:	PCB, Microfluidic chip, Microheater, LAM
Divisions:	Faculty of Electronics and Computer Engineering
Depositing User:	Sabariah Ismail
Date Deposited:	28 Jun 2024 15:59
Last Modified:	28 Jun 2024 15:59
URI:	http://eprints.utem.edu.my/id/eprint/27124
Statistic Details:	View Download Statistic

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